货品编号： Sigma.p4832-50ml 品牌： Sigma 品名： Poly-L-lysine solution 规格： 50ml

产品描述：

Components Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine

to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen

bonding between amino and either the carboxyl groups or N or O containing moieties.

Application Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport,

microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular

weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction

between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions

to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as

the attachment factor.

Preparation Note This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 150 - 300 kDa. Coating slides with this solution should

be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.