货品编号： Sigma.P8811-1G 品牌： Sigma 品名： 蛋白酶 来源于灰色链霉菌 规格： 1G

产品描述：

Other Notes An unusually non-specific protease.

包装 1, 5 g in glass bottle 100 mg in glass bottle

Preparation Note Collected from culture broth of S. griseus.

Quality Contains approximately 25% calcium acetate.

Specificity A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of

substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule.

This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Unit Definition One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu

reagent).

Application Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate

and 10 mM EDTA. This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution.

This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.

Physical properties Completely inactivated by heating above 80 °C for 15-20 minutes.