货品编号：Thermo.AM1921 品牌： Invitrogen 品名： PARIS™ Kit 规格： 50 preps

产品描述：

The Ambion PARIS system is for the isolation of both RNA and native protein from the same experimental sample. The kit also

permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents

for 50 purifications from1 to 75 mg of tissue or 100 to 107 cells each.

• Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent experimental samples

Isolation of high-quality RNA is the first step for a variety of gene expression analyses. Very often, complementary studies at the protein

level are also required. Usually these analyses are performed using different aliquots of the same experimental sample. However, when

working with rare, difficult to obtain, or very small samples, it is sometimes impractical to isolate RNA and native proteins independently.

In studies involving large numbers of samples, expensive reagents, or inherent variability (e.g., cell transfection), the addition of independent

experimental samples is not only costly and time consuming, but may also lead to inconsistent results. To solve these issues, the Protein And

RNA Isolation System (PARIS system) has been developed. It allows for the isolation of both RNA and protein from the same experimental

sample (see Figure). Using the PARIS system, RNA and protein can be isolated simultaneously from whole cell lysates. Alternatively, RNA and

protein can be isolated from separate nuclear and cytoplasmic fractions. Tissue or cultured cells are first homogenized in ice-cold Cell Disruption

Buffer to prepare a total cell lysate. Since the homogenization is performed quickly on ice and in the presence of detergent, both protein and RNA

can be purified directly from this lysate. For RNA isolation, a part of the total cell lysate is immediately mixed with an equal volume of Lysis/Binding

Solution. Total RNA is then purified from the mixture using an RNA binding glass fiber filter. After three rapid washing steps, high-quality RNA is

eluted in a concentrated form. The entire procedure can be completed in less than 20 minutes. Note: This kit is not recommended for tissues with

high levels of ribonucleases, such as pancreas.