货品编号： Invitrogen.fd1464 品牌： Invitrogen 品名： FastDigest BshTI 规格：20 reactions

产品描述

Thermo Scientific FastDigest BshTI restriction enzyme recognizes A^CCGGT site and cuts best at 37°C in 5–15 minutes using universal

FastDigest Buffer. Isoschizomers: AgeI, AsiGI, CspAI, PinAI.

Thermo Scientific FastDigest BshTI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest

and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or

subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and

incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase,

alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications

can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction

products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.