产品描述：

Biochem/physiol Actions Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether)

- N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated

by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in

molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in

tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from

0.1 to 5 mg/mL are used for digestion. The duration of the reaction varies from 15 minutes to several hours to yield a satisfactory

efficiency of cell dissociation without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preffered and Zn2+ is

required for activity. This enzyme is tested for the release of hepatocytes at approximately 1 mg/mL concentration, in a total volume of

100 mL for each rat liver.

Preparation Note A stock solution may be prepared by dissolving 0.05-0.1 mg/mL of collagenase in 50 mM TES buffer containing 0.36 mM calcium chloride

(TESCA), pH 7.4, at 37 °C.

Quality 也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。