货品编号： Invitrogen.17-4801-82 品牌： Invitrogen 品名： F4/80 Monoclonal Antibody (BM8), APC, eBioscience™ 规格： 100ug

产品描述：

Product Specific Information Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs

to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane

spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen

is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types,

such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as

well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro

with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.

Applications Reported: BM8 has been reported for use in flow cytometric analysis.

Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can

be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume

of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody

be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.