货品编号： Invitrogen.11-9668-80

品牌：Invitrogen

品名：anti-mouse Ly6G

规格：25ug

产品描述：

Product Specific Information Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI

protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration.

Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for

Ly-6G is currently unknown.

Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been

used to deplete neutrophils and reported to have antagonist activity.

Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used

at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume

of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the

antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Background/Target Information Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called

polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments.

In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of

the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.