货品编号： Invitrogen.23263 品牌： 23263 品名：" Pierce™ Colorimetric Protease Assay Kit " 规格： 250 tests

产品描述：

The Thermo Scientific Pierce Colorimetric Protease Assay Kit measures total protease activity in samples, providing a means

to assess the progress of protease isolation procedures or quantify protease contamination in biological samples. This kit uses

fully succinylated casein as a substrate for the assay. Hydrolysis of this substrate in the presence of protease results in the

release of peptide fragments with free amino-terminal groups. These peptides are reacted with trinitrobenzene sulfonic acid

(TNBSA), followed by measurement of the absorbance increase that results from the formation of yellow colored TNB-peptide

adducts.

Features of the Colorimetric Protease Assay Kit:

• Casein substrate—measure activity of any protease that cleaves casein into peptide fragments • Trypsin standard—quantify protease activity relative to trypsin, a universally accepted reference • Sensitive—1000 times more sensitive than assays that use unmodified forms of casein • Simple and fast—test tube and microplate protocols are completed in less than one hour • Homogenous—addition steps only; no separation, transfer or stop steps required • Customizable—easily adapt time, temperature and pH to optimize sensitivity for proteases of interest

Applications: • Assess functional integrity and overall progress of protease purification • Quantify protease contamination in protein samples • Characterize the activity of unusual proteases compared to trypsin • Evaluate buffer conditions for their affects on protease function and activity

Additional Specifications: • Substrate: succinylated casein (not available separately) • Basis of assay: detection of primary amines resulting from proteolysis • Detection reagent: trinitrobenzene sulfonic acid (TNBSA) • Measurement: absorbance at 450nm (plate reader or spectrophotometer)