产品描述

Analysis Note The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be

made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated

by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.

Preparation Note A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc.

It should be stored at 4 °C.

Unit Definition One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by

Folin-Ciocalteu reagent), unless otherwise indicated.