货品编号： Thermo.61-5931-82 品牌：Thermo 品名：Anti-Mouse Ly-6G (Gr-1) PE-eFluor® 610 规格：100 ug

产品描述：

Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid

differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated

manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the

expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells

that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G

(cat. 9668) be used when looking at Ly-6G specific targets.

Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This

can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample

in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is

recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band

pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step

Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined

empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.