Beta葡聚糖酶[麦芽和微生物]检测试剂盒

英文名：β-Glucanase Assay Kit (Malt and Microbial)

货号：K-MBGL

规格：100 assays per kit

β-葡聚糖酶作用方式不同，可分为内切型和外切型。前者存在于谷物种子、某些真菌和某些细菌中，能催化水解谷物细胞壁中的β-葡聚糖，其中包括内切型β-1,4-葡聚糖酶、内切型β-1,3-葡聚糖酶。后者存在于谷物种子中，其中又包括外切型β-1,4-葡聚糖酶、外切型β-1,3-葡聚糖酶。 在工业上采用细菌（如枯草芽孢杆菌）、曲霉（如黑曲霉）、青霉（如伊氏青霉）生产。在啤酒生产原料大麦及其麦芽中，含本品较多。在啤酒生产中采用β-葡聚糖酶，可提高麦汁的过滤速度和得率，从而保证啤酒质量。也可用于饴糖、麦芽糖浆生产和用作饲料添加剂。

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.

Colourimetric method for the determination of β-Glucanase in malt, foodstuffs and fermentation products

Principle: (β-glucanase) (1) Azo-Barley β-glucan (polymer) → Azo-barley β-glucan (fragments)

(2) Add alcohol; centrifuge to remove polymeric Azo-barley β-glucan

(3) Measure the absorbance of the supernatant solution

Kit size: 100 assays Method: Spectrophotometric at 590 nm Reaction time: ~ 30 min Detection limit: 100 U/kg of malt Application examples: Malt extracts, wort, beer and other materials Method recognition: RACI (Standard Method)

Advantages

• Very cost effective
• All reagents stable for > 2 years during use
• Only kit available
• Very specific
• Simple format
• Standard included