货品编号： Thermo.A-21200 品牌：Thermo 品名： Chicken anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 规格：500ul 产品描述：

Product Specific Information To minimize cross-reactivity, these chicken anti-mouse IgG (H+L) whole secondary antibodies have been affinity purified

and cross-adsorbed against human and rabbit IgG prior to conjugation. Cross-adsorption or pre-adsorption is a purification

step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary

antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive

species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries

flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry)

where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where

there are may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright,

green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow

cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and

high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules

can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more

sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the

exact degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the

supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage,

thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution

of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should

be satisfactory for most immunohistochemistry and flow cytometry applications.