货品编号： thermo.21127022 品牌：thermo 品名：Ham's F-12K (Kaighn's) Medium 规格：500ml 产品描述：

Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium

was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum

environment.

This F-12K (Kaighn's) Medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red

The complete formulation is available.

Using Ham's F-12K Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine,

thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the

medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K

(Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and

Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium

uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain

physiological pH.