wako125-05061赖氨酰肽链内切酶 Lysyl Endopeptidase 【产品详情】 赖氨酰肽链内切酶 品牌：WAKO和光 级别：质谱级 赖氨酰肽链内切酶Lysyl Endopeptidase 125-05061 wako 英文品名：Lysyl Endopeptidase?, Mass Spectrometry Grade 货号：125-05061 规格：5×20 μg 应用：蛋白质组学。 用途：赖氨酰肽链内切酶，蛋白测序、质谱分析，多肽合成 赖氨酰肽链内切酶Lysyl Endopeptidase 125-05061 wako 【实验原理】 赖氨酰肽链内切酶是质谱分析前处理时最常用的蛋白分解酶即赖氨酰肽链内切酶，赖氨酰肽链内切酶可以特异性切除赖氨酸基团C末端的多肽，赖氨酰肽链内切酶可用于蛋白测序分析和Lys-X化合物的酶合成。 若同时使用赖氨酰肽链内切酶和胰酶，可更好地切断赖氨酸基团的多肽，增加多肽的数量。赖氨酰肽链内切酶已按照使用习惯做成小包装，是方便使用赖氨酰肽链内切酶的冷冻干燥品。 【产品特点】 赖氨酰肽链内切酶Lysyl Endopeptidase 125-05061 wako 1）赖氨酰肽链内切酶用于蛋白质谱分析：高特异性、高蛋白质消化率； 2）赖氨酰肽链内切酶能增加肽段数量、提高裂解效率； 3）赖氨酰肽链内切酶独立包装，20 μg/一支 【结果】表明Lep酶解的错误裂解率最低。 Tp酶解时加入Lep后，错误裂解率有所降低，同时，赖氨酰肽链内切酶可以鉴定出更多的多肽。

Tp Lep Lep +Tp 裂解位点 精氨酸和赖氨酸的C端 赖氨酸的C端 精氨酸和赖氨酸的C端 错误裂解率(错误裂解所占比例) 多(8 %) 很少(0 %) 少(3 %) 鉴定出的多肽数量 17 19 22

赖氨酰肽链内切酶Lysyl Endopeptidase 125-05061 wako Lep+Tp赖氨酰肽链内切酶切后，可以在m/z=2000时得到吸收峰，而单独的Tp酶切在m/z=2000时没有吸收峰。该结果表明Lep可以提高测序覆盖度。 赖氨酰肽链内切酶Lysyl Endopeptidase 125-05061 wako

WAKO 其他产品清单：

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

货号品名规格 Cas

012-01965 Alminium Oxide 500g –

012-08643 Amino Acids Mixture Standard Solution, Type B 1mL×5A –

013-08391 Amino Acids Mixture Standard Solution, Type H 5mL –

015-14461 Amino Acids Mixture Standard Solution, Type AN-2 5mL –

015-25191 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) 50ul –

011-14463 Amino Acids Mixture Standard Solution, Type AN-2 1mL×5A –

011-19365 2,2′-Azobis[2-(2-imidazolin-2-yl)propane]

Dihydrochloride 500g 27776-21-2

016-08641 Amino Acids Mixture Standard Solution, Type B 5mL –

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

016-20001 Anti Iba1, Rabbit (for Western Blotting) 50μg –

016-23281 Anti Rat P2X4, Monoclonal Antibody 50ug –

017-19362 2,2′-Azobis[2-(2-imidazolin-2-yl)propane] Dihydrochloride 25g 27776-21-2

018-08721 Agar, Powder 100g 9002-18-0

018-22021 Anti Mouse Ago2, Monoclonal Antibody 100μL –

019-03435 Ammonium sulfate 500g 7783-20-2

019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 50μg –

019-22291 Anti Olfactory Marker Protein, Goat 100μL –

021-02195 Boric acid 500g 10043-35-3

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

021-16201 BES-H2O2(Cell-impermeant) 1mg –

031-17601 Collagenase Type I 100mg 9001-12-1

032-08385 Citric Acid Monohydrate 500g 5949-29-1

032-15372 Casein Phosphopeptide 25g –

034-06363 N-Caprylic Acid 25mL 124-07-2

034-16111 Cefotaxime Sodium Salt 1g 64485-93-4

035-04595 Creatinine 500g 60-27-5

035-17604 Collagenase Type I 1g 9001-12-1

038-23221 α-Casein, from Bovine Milk, Dephosphorylated 1mg –

039-01335 Carboxymethyl Cellulose Sodium Salt 500g 9004-32-4

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

021-17801 BES-So-AM 1mg 936356-51-3

022-07985 γ-Butyrolactone 500mL 96-48-0

025-03195 Buffer Solution Standard (Phosphate pH Standard Equimolal Solution)

pH6.86 (25 degrees C) 500mL –

047-18863 Dexamethasone 100mg 50-02-2

049-18801 4′,6-Diamidino-2-phenylindole Dihydrochloride n-Hydrate 10mg 28718-90-3

058-07681 EasySeparator 1set –

025-03195 Buffer Solution Standard (Phosphate pH Standard Equimolal Solution)

pH6.86 (25 degrees C) 500mL –

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

019-08393 Amino Acids Mixture Standard Solution, Type H 5Ax1mL –

019-11941 Active Carbon-impregnated Silica Gel 10g –

019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 50μg –

025-15481 BES-Thio 1mg –

026-16371 BF-170 1mg –

027-01276 Benzyl Alcohol 500mL 100-51-6

028-03185 Buffer Solution Standard (Phthalate pH Standard Solution)

pH4.01 (25 degrees C) 500mL –

028-03185 Buffer Solution Standard (Phthalate pH Standard Solution)

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

pH4.01 (25 degrees C) 500mL –

028-03205 Buffer Solution Standard (Tetraborate pH Standard Solution)

pH9.18 (25 degrees C) 500mL –

077-03155 Gelatin 500g 9000-70-8

081-00136 Heparin Sodium 10mu 9041-08-1

088-00705 L-Histidine Hydrochloride Monohydrate 500g 5934-29-2

093-06471 Insulin, Human, recombinant 50mg –

097-06474 Insulin, Human, recombinant 1g 11061-68-0

120-04891 L-012 100mg –

125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 5×20μg –

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

129-02541 Lysyl Endopeptidase( R) 10AU – –

129-04861 LY 294002 5mg 154447-36-6

130-07871 Melibiose 5g 585-99-9

028-16211 BES-So (Cell-impermeant) 1mg –

028-17811 BES-H2O2-Ac 1mg –

029-16361 BF-168 1mg –

039-23511 CultureSure? Freezing Medium 100ml –

041-18861 Dexamethasine 1g 50-02-2

042-29445 Dibasic Sodium Phosphate Hydrate 500g 10039-32-4

043-22611 Dextran 200,000 100g 9004-54-0

064-05381 Fibroblast Growth Factor (basic)(FGFb / bFGF / FGF2),

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

Human, recombinant, Animal-derived-free 50ug 106096-93-9

071-02293 gamma-Globulin, from Human Serum 5g 9007-83-4

075-03075 Gellan Gum 500g 71010-52-1

131-00405 Magnesium Sulfate 500g 10034-, 99-8

136-03032 Methyl Red 25g 493-52-7

137-06085 Molecular Sieves 4A1/16 500g 70955-01-0

138-07872 Melibiose 25g 585-99-9

145-08221 Nuclease P1 500u 54576-84-0

145-08221 Nuclease P1 500u 54576-84-0

162-09115 Polyethylene Glycol 4,000 500g 25322-68-3

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

163-03545 Potassium Chloride 500g 7447-40-7

164-21655 N/400 Potassium Polyvinyl Sulfate Solu 500mL 26837-42-3

191-01785 Trisodium Citrate Dihydrate 500g 6132-04-3

193-02041 Glycerol 2-Phosphate Disodium Salt n-Hydrate 100g 819-83-0

195-12854 Streptavidin, from Streptomyces avidinii 5mg 9013-20-1

195-16031 StemSure (R) Freezing Medium 100ml –

196-00015 Sucrose 500g 57-50-1

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

196-08675 Sodium Dodecyl Sulfate 500g 151-21-3

197-07485 Sodium Sulfate 500g 7757-82-6

199-02825 Sodium Dihydrogenphosphate Dihydrate 500g 13472-35-0

203-17561 Trichostatin A 1mg 58880-19-6

207-00055 L(+)-Tartaric Acid 500g 87-69-4

208-08385 1mol/l Triethylammonium Hydrogencarbonate Solution 500mL 15715-58-9

223-02112 VA-044 25g 27776-21-2

225-02111 VA-044 100g 27776-21-2

165-09105 Polyethylene Glycol 2,000 500g 25322-68-3

165-17035 Polyvinylpyrrolidone K 30 500g 9003-39-8

167-09045 Polyethylene Glycol 200 500g 25322-68-3

169-09125 Polyethylene Glycol 6,000 500g 25322-68-3

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

185-01003 Rifampicin 5g 13292-46-1

186-01114 all-trans-Retinoic Acid 50mg 302-79-4

189-01001 Rifampicin 1g 13292-46-1

190-05535 Trisodium Citrate Dihydrate 500g 6132-04-3

191-01665 Sodium Chloride 500g 7647-14-5

227-01071 Valproic Acid 5g 99-66-1

231-02011 Wetting Tension Test Mixture No.52.0 50mL –

232-01941 Wetting Tension Test Mixture No.42.0 50mL –

233-01971 Wetting Tension Test Mixture No.45.0 50mL –

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

234-02001 Wetting Tension Test Mixture No.50.0 50mL –

235-01931 Wetting Tension Test Mixture No.41.0 50mL –

235-02031 Wetting Tension Test Mixture No.56.0 50mL –

236-01961 Wetting Tension Test Mixture No.44.0 50mL –

238-01921 Wetting Tension Test Mixture No.40.0 50mL –

238-02021 Wetting Tension Test Mixture No.54.0 50mL –

238-02141 Wetting Tension Test Mixture No.73.0 50mL 7732-18-5

253-00513 Y-27632 5mg 331752-47-7

257-00511 Y-27632 1mg 331752-47-7

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

290-34251 Presep-C DNPH 20EA –

296-63801 LabAssay? Phospholipid 1300tests –

298-64101 Fructooligosaccharides standard set set –

302-93561 Phos-tag (TM) Agarose 0.5mL –

304-93521 Phos-tag (TM) Acrylamide AAL-107 10mg –

304-93521 Phos-tag (TM) Acrylamide AAL-107 10mg –

290-34251 Presep-C DNPH 20EA –

290-63701 LabAssay? Triglyceride 1000tests -2016-2-29

290-65901 LabAssay? Creatinine 500tests –

291-58601 Lab Assay ALP 900tests –

293-51601 Endotoxin Extracting Solution for LAL Test 10mL x 4 –

294-62001 DsDD cDNA Subtraction kit Wako 5tests –

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

294-63601 LabAssay? NEFA 750tests –

294-65801 LabAssay? Cholesterol 1000tests –

295-50201 DNA Extractor? Kit 50tests –

295-51301 Limulus ES-II Single Test Wako 25 tests –

295-51301 Limulus ES-II Single Test Wako 25 tests –

295-73401 Fructooligosaccharides Set Set –

304-93526 Phos-tag (TM) Acrylamide AAL-107 5mM Aqueous Solution 0.3ml –

305-93551 Phos-tag? Mass Analytical Kit set –

308-93541 Phos-tag (TM) Biotin BTL-105 10mg –

308-93563 Phos-tag (TM) Agarose 3mL –

308-97201 Phos-tag Biotin BTL-1111 1mM Aqueous Solution 0.1000mL –

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

325-88521 Lipase PS Amano SD 10g –

298-65701 LabAssay? Glucose 1000tests –

300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg –

301-93531 Phos-tag (TM) Biotin BTL-104 10mg –

301-99413 Phos-tagTM Biotin BTL-104 1mM Aqueous Solution[for Campaign] 0.1mL 753451-66-0

302-93522 Phos-tagTM Acrylamide AAL-107 5mM Aqueous Solution[for Campaign] 0.3mL 871839-54-2

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货

631-00651 Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) 20mL –

637-00653 Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) 100mL –

637-00653 Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) 100mL –

638-01021 Endotoxin Standad /SGGGOE 10000un –

986-10001 Anti asialo GM1 (Rabbit), (014-09801) 1mL 617-45-8

Wako125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade现货