货品编号： Gibco.11965118 品牌：Gibco 品名：DMEM, high glucose 规格：10 x 500 mL

产品描述：

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many

different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells,

HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies

offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the

media selector tool.

This DMEM is modified as follows:

With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Phenol Red

The complete formulation is available.

Using DMEM DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the

original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium

pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins,

lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to

maintain physiological pH.