Harlan肥胖模型高酯饲料 Diet induced obesity Harlan Teklad动物饲料 Harlan代理 欢迎新老客户访问Harlan官网或者咨询中国代理商金山科研平台获取更多详细资料。 Harlan肥胖高酯模型 Diet induced obesity Purified high fat diets used to induce obesity and obesity-related complications such as diabetes and metabolic syndrome typically have 40-60% of energy derived from fat. The diet tables below summarize relevant diet features for several Teklad custom research diets commonly used in rodent models. Teklad also creates high-fat diets for other species, including pigs, primates, and dogs. Contact us to discuss the use of these diets or one that better meets your needs.

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 55-60% of calories from fat Diet features TD.06414stocked TD.93075dough TD.07011pellet Kcal/g 5.1 4.8 Fat, % Kcal 60 55 Fat Sources, % by weight 31% lard 3% soybean oil 27.4% vegetable shortening 1.6% corn oil Fatty acid profile, % total fat 37% saturated 47% monounsaturated 16% polyunsaturated 28% saturated, 30% trans 28% monounsaturated (cis) 14% polyunsaturated (cis) Sucrose, % by weight 12.1 9.6 Notes 60F10S poster data Compare to D12492 Trans fat Example modifications TD.08500coconut oil TD.09766milk fat Ingredient matched, low fat control diets* TD.06416(35% sucrose) TD.08806(11% sucrose) TD.93074(21% sucrose) TD.120651(7% sucrose) References Mouse Rat Mouse Rat

*Control diets can be designed in several ways, depending on what features the researcher wants to modify relative to the high-fat diet. These are just a few examples.

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 40-45% of calories from fat Diet features TD.06415 TD.08811 TD.88137stocked TD.95217 Kcal/g 4.6 4.7 4.5 4.3 Fat, % Kcal 45 45 42 40 Fat sources, % by weight 19.5% lard 3% soybean oil 21% milk fat 2% soybean oil 21% milk fat 10.6% vegetable shortening 4% milk fat 4% soybean oil Fatty acid profile, % total fat 36% saturated 46% monounsaturated 18% polyunsaturated 61% saturated 31% monounsaturated 8% polyunsaturated 62% saturated 27% monounsaturated 5% polyunsaturated 34% saturated, 18% trans 29% monounsaturated (cis) 19% polyunsaturated (cis) Sucrose, % by weight 22.8 36.8 34.5 15.8 Notes Compare to D12451 45F30S poster data “Western Diet” Cholesterol added Trans fat Example modifications TD.110716milk fat TD.10670no dye TD.130784lard TD.120438no dye TD.07201lard TD.00573h-coconut oil, no cholesterol TD.09682blue dye TD.07734green dye Ingredient matched, low fat control diets* TD.06416(35% sucrose) TD.110675(18% sucrose) TD.120455(6% sucrose, resistant starch) TD.120724(14% sucrose) TD.05230(34% sucrose) TD.08485(12% sucrose) TD.06101(6% sucrose) References Mouse Rat Mouse Rat Mouse Rat Mouse Rat 饮食诱导肥胖

用于诱导肥胖和肥胖相关并发症(如糖尿病和代谢综合征)的纯化高脂饮食通常有40-60%的能量来源于脂肪。下面的饮食表总结了一些特克拉德定制研究饮食的相关饮食特征，这些研究饮食通常用于啮齿类动物模型。 泰克乐还为其他物种，包括猪、灵长类动物和狗创造高脂肪饮食。联系我们讨论使用这些饮食或更好地满足您的需要。

通常使用的饮食诱导肥胖(DIO)技术啮齿动物饮食中含有55-60%的热量来自脂肪。 饮食特征 TD.06414备好 TD.93075面团 TD.07011球团 千卡/克 5.1 4.8 脂肪，%千卡 60 55 脂肪来源， 按重量计百分比 31%猪油 3%豆油 27.4%蔬菜酥油 1.6%玉米油 脂肪酸谱， 总脂肪百分比 37%饱和 47%单不饱和 16%多不饱和 28%饱和，30%反式 28%单不饱和(顺式) 14%多不饱和 蔗糖 按重量计百分比 12.1 9.6 注记 60F10S海报数据 与D 12492比较 反式脂肪 示例修改 TD.08500椰子油 TD.09766乳脂 成分匹配， 低脂控制饮食* TD.06416(35%蔗糖) TD.08806(11%蔗糖) TD.93074(21%蔗糖) TD.120651(7%蔗糖) 参考文献 鼠 大鼠 鼠 大鼠

*控制饮食可以通过几种方式来设计，这取决于研究人员相对于高脂肪饮食想要改变什么特征。这些只是几个例子。

通常使用的饮食诱导肥胖(DIO)技术啮齿动物饮食中含有40-45%的热量来自脂肪。 饮食特征 TD.06415 TD.08811 TD.88137备好 TD.95217 千卡/克 4.6 4.7 4.5 4.3 脂肪，%千卡 45 45 42 40 脂肪来源， 按重量计百分比 19.5%猪油 3%豆油 21%乳脂 2%豆油 21%乳脂 10.6%蔬菜酥油 4%乳脂 4%豆油 脂肪酸谱， 总脂肪百分比 36%饱和 46%单不饱和 18%多不饱和 61%饱和 31%单不饱和 8%多不饱和 62%饱和 27%单不饱和 5%多不饱和 34%饱和，18%反式 29%单不饱和(顺式) 19%多不饱和 蔗糖 按重量计百分比 22.8 36.8 34.5 15.8 注记 与D 12451比较 45F30S海报数据 “西餐” 添加胆固醇 反式脂肪 示例修改 TD.110716乳脂 TD.10670无染料 TD.130784猪油 TD.120438无染料 TD.07201猪油 TD.00573椰子油，不含胆固醇 TD.09682蓝色染料 TD.07734绿色染料 成分匹配， 低脂控制饮食* TD.06416(35%蔗糖) TD.110675(18%蔗糖) TD.120455(6%蔗糖，抗性淀粉) TD.120724(14%蔗糖) TD.05230(34%蔗糖) TD.08485(12%蔗糖) TD.06101(6%蔗糖) 参考文献 鼠 大鼠 鼠 大鼠 鼠 大鼠 鼠 大鼠

Diets for diet-induced obesity (DIO) Diets with 55-60% of calories from fat likeTD.06414andTD.93075are commonly used for inducing obesity in rodents. While considered extreme compared to typical human fat consumption, these diets are effective in initiating rapid weight gain in most rodents. With higher fat content there is less room for carbohydrate, thus the carbohydrate (particularly sucrose) amount is relatively low compared to other obesity inducing diets. If you are interested in high fat and high carbohydrate, look at diets with 40-45% of calories from fat (often referred to as western diets). As the fat level increases, pellet quality (durability) is often compromised. Some higher fat formulas are available only in non-pelleted form or require specific carbohydrate, maltodextrin, for pelleting. Depending on the fat and carbohydrate sources used, the non-pelleted form could be dense and crumbly, dough-like, or paste-like. Though a little more challenging to work with, non-pelleted diet is still used by many researchers for diet-induced obesity models as these researchers suspect the softer form may enhance obesity development. Diets with 40-45% of calories from fat, likeTD.95217,TD.88137,TD.06415, andTD.08811, represent another popular diet pattern for diet-induced obesity work. These diets have double or triple the amount of sucrose found in higher fat diets. High levels of simple carbohydrate like sucrose and fructose may help to promote hypertriglyceridemia, insulin resistance, and fatty liver. Diets with a pattern of high sucrose and high saturated or trans fat are often referred to as “Western Diets” in obesity and cardiovascular fields. Some “Western Diets” have further modifications to the fatty acid profile or even specific vitamin and minerals adjustments to be even more closely matched to a Western Diet pattern. For specific fatty acid modifications, see examples on ourfat/lipid adjusted dietspage. Diets for diet-induced diabetes Many of the same diets used for inducing obesity in rodents can be used to enhance diabetes related phenotypes like insulin resistance and glucose intolerance. However, fasting hyperglycemia characteristic of diabetes (glucose > 200 mg/dL) is uncommon with a diet only approach. Pre-feeding a high fat diet to induce a certain level of obesity and insulin resistance and then giving low-dose streptozotocin (STZ) may be an effective approach if overt hyperglycemia is desired. Ingredient matched, low-fat DIO control diets There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined, simple digestion) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible). A very basic purified control diet would be AIN-93M (TD.94048) or AIN-93G (TD.94045). AIN-93 diets have a moderate amount of sucrose at ~10%, and fat is from soybean oil with a healthy fatty acid profile. Additional examples of controls for specific DIO diets can be found in the above tables. Many researchers choose to compare their high fat fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diets or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens). Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component.