货品编号： Invitrogen.A2910002 品牌： Invitrogen 品名：ExpiCHO™ Expression Medium 规格： 6 x 1 L

产品描述：

ExpiCHO™ Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal

origin–free medium specifically designed for high-density growth and transfection of suspension-adapted

Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO™ Expression System Kit (Cat.

No. A29133) for scalable transient protein production.

ExpiCHO Expression Medium: • Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability • Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies

of >90% with ExpiFectamine™ CHO transfection reagent • Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL • Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to

3 g per L of recombinant protein • Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop

bioreactors • Does not contain phenol red

ExpiCHO Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required.

The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein

expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which is supplied as part of

the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent

cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO2 cell culture incubator.

However, cultures are scalable from volumes of <1 mL in multi-well plates to >10 L in bioreactors.