货品编号： Thermo.56-9459-42 品牌：Thermo 品名：CD45 Monoclonal Antibody （2D1），Alexa Fluor 700, eBIOSCENCETM 规格： 100tests

产品描述：

Product Specific Information

Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common

Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The

cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of

lymphocytes.

Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human

peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that

will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from

10^5 to 10^8 cells/test.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP

mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.