货品编号： Thermo.25-4031-82 品牌：Thermo 品名：Rat IgG2b kappa Isotype Control, PE-Cyanine7, eBioscience 规格：100 µg

产品描述：

Product Specific Information Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.

Applications Reported: PE-Cy7 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.

Applications Tested: Rat IgG2b K Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of

mouse splenocytes. It should be used at the same concentration as the experimental antibody.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step

Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET

efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific

performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.