货品编号： Thermo.50-4801-82 品牌：Thermo 品名：F4/80 Monoclonal Antibody (BM8), eFluor 660, eBioscience™ 规格：100ug

产品描述：

Product Specific Information Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor.

It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus,

seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7

superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers

for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other

dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early

myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of

macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.

Applications Reported: This BM8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This BM8 antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate

cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a

cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8

cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm).

Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.