货品编号： Thermo.25-9985-82 品牌：Thermo 品名：CD279 (PD-1) Monoclonal Antibody (J43), PE-Cyanine7, eBioscience™ 规格：100ug

产品描述： Product Specific Information Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the

Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in

peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes.

Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence

reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal

tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse

PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43

are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells.

Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Applications Reported: This J43 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This J43 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes.

This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a

cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8

cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step

Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET

efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific

performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.