货品编号: Thermo.AM9690 品牌:Thermo 品名:Plant RNA Isolation Aid 规格:10mL
产品描述: Plant RNA Isolation Aid is recommended for use with the RNAqueous Kit or MagMAX Plant RNA Isolation
Kit for purification of total RNA from plant tissues.
• Removes common plant contaminants such as polyphenolics and polysaccharides • No phenol extraction, alcohol precipitation, or proteinase digestion steps required when used with the recommended kits
Plant RNA Isolation Aid contains polyvinylpyrrolidone (PVP), a high molecular weight polymer that binds to contaminants
such as polyphenolics and polysaccharides that are commonly present in plant tissues. Glass fiber filter–based isolation,
employed by the RNAqueous Kit and MagMAX Plant RNA Isolation Kit, is especially suited for plant RNA isolation because
it does not include any alcohol precipitation steps; many common contaminants found in plant tissue are known to co-precipitate
with nucleic acid during alcohol precipitation. When used with the RNAqueous Kit or MagMAX Plant RNA Isolation Kit, the
procedure consists of disrupting the plant tissue in a guanidinium-based lysis solution to which the Plant RNA Isolation Aid has
been added. A brief centrifugation then removes the contaminants. The supernatant is passed through a glass fiber filter which
binds RNA. The filter is then washed and the RNA is eluted in a small volume (50 µL or more) of elution solution.
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