promega.M7845 GoTaq® G2 DNA Polymerase 500uPromega


货品编号: promega.M7845 品牌:promega 品名:GoTaq® G2 DNA Polymerase 规格:500u

产品描述: 新一代GoTaq® DNA聚合酶,可高效可靠地进行PCR扩增反应 全长Taq DNA聚合酶,带有5´→3´外切酶活性 产物可直接上样进行凝胶电泳 5X 反应缓冲液中含有MgCl2 ,简化了反应体系配制 产品以5u/μl酶浓度提供

GoTaq® G2 DNA Polymerase, the second generation of GoTaq® products reliably amplifies a wide range of PCR templates and

provides high-performing results due to improved manufacturing processes, increased reliability and consistency.GoTaq® G2 is

supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification.

The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity. GoTaq® G2 DNA Polymerase is

supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Both buffers contain MgCl2 at a

concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction. The 5X Green GoTaq® Reaction Buffer has two dyes

(blue and yellow) that separate during electrophoresis to indicate migration progress. The buffer also includes a compound that increases

sample density so that samples can be loaded directly onto gels without the need for loading dye. The colorless buffer is used when direct

fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.

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