货品编号： thermo.17-5071-41 品牌：thermo 品名：BrdU Monoclonal Antibody (BU20A), APC, eBioscience™ 规格：25 Tests

产品描述： Description:

This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA

in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis

during BrdU treatment.

For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (cat. 00-5525) and protocol.

Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This BU20A antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of BrdU-labeled

mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol or the BrdU Staining Buffer Set (cat. 00-5525)

and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B:

One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody

that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.