货品编号: Thermo.N8080241 品牌:Thermo 品名:AMPLITAQ GOLD 25OU BUFFER II EACH 规格:250 units
产品描述: AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation.
Features of this enzyme:
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield • Time-released thermal activation improves sensitivity in low copy number amplifications • Successful multiplex PCR saves time and reagents • Includes GeneAmp® 10X PCR Buffer II with MgCl2
Hot-start activation The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating
active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional
PCR techniques.
Higher specificity, higher yield AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate
slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation
step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of
specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA
Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.
AmpliTaq Gold® 360 DNA Polymerase for superior performance Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase,
AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA
sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start
capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.
Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
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