SIGMA.F9037 Formamide 100mlSigma-Aldrich


货品编号: SIGMA.F9037 品牌:SIGMA 品名:Formamide 规格:100ml

产品描述: Application Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols

requiring lower hybridization temperatures.

Formamide is suitable for use in hybridization buffers made using standard recipes. It was used in preparation of brain sections

of mice for BrdU immunohistochemistry[2] and FISH analysis of HeLa cells.[3] It was used to prepare the polyacrylamide gel for

analysis of PCR products using denaturing gradient gel electrophoresis.[1]

General description Formamide is a clear liquid amide derived from formic acid. It can be used as a cryoprotectant and gel-stabilizer. It is known to

destabilize nucleic acid duplexes, making it useful for preparing hybridization buffers for RNA and DNA applications.

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