货品编号：thermo.53-9892-82

品牌：thermo

品名：GFAP Monoclonal Antibody (GA5), Alexa Fluor 488, eBioscience™

规格：100ug

产品描述：

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig

glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed

in neural tissues and distinguishes astrocytes from other glial cells during central nervous system

development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant

form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such

associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent

on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that

mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be

overexpressed in certain glial-derived tumors.

Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow

cytometric analysis, immunohistology staining of frozen and FFPE tissue sections, and immunocytochemistry.

Applications Tested: This GA5 antibody has been tested by immunocytochemistry of fixed and permeabilized

C6 cells and by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH

antigen retreival. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody

be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.