Gibco™ RPMI 1640 Medium, powder

简要描述：Gibco™ RPMI 1640 Medium, powder was originally developed to culture human leukemic cells in suspension and as a monolayer.

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Gibco™ RPMI 1640 Medium, powder

Using RPMI：

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP manufacturing and quality system：

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.