货品编号： thermo.17-5932-82

品牌： thermo

品名： Ly-6C Monoclonal Antibody (HK1.4), APC, eBioscience

规格： 100ug

产品描述：

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked

protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells

and granulocytes as well as a subset of lymphocytes. Some variation of expression is

found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes.

These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on

C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2

found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.

In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release.

Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse

splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the

amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number

should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended

that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.