thermo.A-21206 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 1mgInvitrogen


货品编号: thermo.A-21206 品牌:thermo 品名:Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 规格:1mg

产品描述: To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show

minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep

serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody

resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a

column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-

binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The

benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where

there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where

there may be the presence of endogenous immunoglobulins.

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