Invitrogen.A-11003 Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 500 µLInvitrogen


货品编号: Invitrogen.A-11003 品牌:Invitrogen 品名:Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 规格:500 µL

产品描述: To minimize cross-reactivity, these goat anti-mouse IgG (H+L) whole secondary antibodies have been affinity purified and

cross-adsorbed against human IgG and human serum prior to conjugation. Cross-adsorption or pre-adsorption is a purification

step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody

solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only

the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The

benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is

potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be

the presence of endogenous immunoglobulins.

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