货品编号： apexbio.A3472

品牌： apexbio

品名： Hoechst 33342蓝色荧光染料

规格： 25mg

产品描述：

Description: IC50 Value: N/A Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.

These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so

that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst

stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342

are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited

by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum

at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes

are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations

can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when

protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the

minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although

the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably.

Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often

called supravital, which means that cells survive a treatment with these compounds. Cells that express specific

ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm. in vitro:

N/A in vivo: N/A Clinical trial: N/A