Thermo.a21208 Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 1mgThermo


货品编号: Thermo.a21208

品牌: Thermo

品名: Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

规格: 1mg

产品描述:

These donkey anti-rat IgG (H+L)whole secondary antibodies have been affinity-purified and show minimum

cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, and sheep

serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody

resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through

a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the

nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries

flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments

(e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell

fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

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