品牌： Invitrogen

品名： IL-1 beta Mouse Uncoated ELISA Kit with Plates

规格： 2*96T

产品描述：

The Mouse Interleukin-1beta (IL-1b) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for

performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse

IL-1b. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

Principle of the method

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific

antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or

controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the

addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target

complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target

present in the original specimen.