品牌： Invitrogen

品名： TNF alpha Mouse Uncoated ELISA Kit with Plates

规格： 2*96T

产品描述：

The Mouse Tumor Necrosis Factor Alpha (TNF-α) Uncoated ELISA Kit contains pre-matched antibody pairs, plates

and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein

levels of mouse TNF-α. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

Principle of the method

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific

antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls

are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the

second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce

measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original

specimen.