货品编号： thermo.A53225 品牌：thermo 品名：Pierce™ Rapid Gold BCA Protein Assay Kit 规格：500ml

产品描述：

The Pierce Rapid Gold BCA Protein Assay Kit is a fast, two-component, high-precision, detergent-compatible assay

optimized to determine total protein concentration. It uses the same copper-chelating technology as the well-known

traditional Pierce BCA Protein Assay and provides comparable accuracy but with a 5-min, room temperature incubation

and measured at 480 nm. This improvement eliminates the need to wait or expose the samples to elevated temperatures

for a fast time to results.

Features of the Rapid Gold BCA Protein Assay Kit include: •Absorbance—colorimetric assay, with optimal absorbance at 480 nm •Uniformity—exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein

variation than dye-binding protein assay methods (Bradford) •Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents •Assay time—5-min, room temperature reaction •Assay range—linear working range for BSA of 20 to 2000 µg/mL

The traditional BCA Protein Assay is a widely used and accepted protein assay, trusted for its highly accurate protein

concentration determination and compatibility with most sample types encountered in protein research. However, in order to

develop the samples in this traditional protocol, one must either heat the reaction at 37°C for 30 minutes or allow room temperature

development for two hours. The Rapid Gold BCA Protein Assay maintains the key characteristics of the traditional BCA assay but

allows a fast time and room temperature incubation equal to dye-binding methods. The Rapid Gold BCA assay can be used to assess

yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared

to most dye-binding methods and similar to the traditional BCA assay, the Rapid Gold BCA assay is affected much less by protein

compositional differences, providing low protein-to-protein variation.