货品编号: Thermo.11550043 品牌:Thermo 品名:gibco Ham's F-10 Nutrient Mix 规格:500 mL/支
产品描述: Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been
used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary
rat astrocytes, and rat prostate epithelial cells.
This Ham's F-10 is modified as follows:With Without • Phenol Red • HEPES • L-glutamine
The complete formulation is available.
Using Ham's F-10 Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth
of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or
growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate
buffer system (1.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system F-10 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device
manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical F-10 product made in our Scotland facility
(31550-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
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