品牌： Bioss

品名： Anti-caspase-3 p12 subunit活化半胱胺酸蛋白酶蛋白3抗体

规格：100ul

研究领域 细胞生物 免疫学 信号转导 细胞凋亡

抗体来源 Rabbit

克隆类型 Polyclonal

交叉反应 Human, Mouse, Rat, Dog, Rabbit,

产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 12/32kDa

细胞定位 细胞浆

性 状 Liquid

浓 度 1mg/ml

免 疫 原 KLH conjugated synthetic peptide derived from human caspase-3 p12 subunit:201-277/277

亚 型 IgG

纯化方法 affinity purified by Protein A

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMed PubMed

产品介绍 The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively

studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is

processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10).

The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme.

Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases

(e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two

transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be

widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts).

Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long

lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which

is associated with neuronal death in Alzheimer's disease.