货品编号： Bioss.bs-2593R 品牌：Bioss 品名：Anti-Caspase 3 precursor半胱天冬酶-3酶原抗体 规格：100ul

研究领域 细胞生物神经生物学信号转导细胞凋亡 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human,Mouse,Rat,Dog,Rabbit, 产品应用 WB=1:500-2000ELISA=1:500-1000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量 28kDa 细胞定位 细胞浆 性状 Liquid 浓度 1mg/ml 免疫原 KLH conjugated synthetic peptide derived from human Caspase 3 precursor:11-120/277 亚型 IgG 纯化方法 affinity purified by Protein A 储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed

产品介绍

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is

composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-

receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to

liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama,

CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa

form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6,

etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small

subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.