货品编号： Bioss.bs-3471R 品牌：Bioss 品名：Anti-Phospho-Vimentin (Ser56)磷酸化波形蛋白抗体 规格：100ul

产品类型 磷酸化抗体 研究领域 肿瘤细胞生物免疫学发育生物学神经生物学信号转导干细胞细胞骨架肿瘤细胞生物标志物 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human,Mouse,Rat,Dog,Pig,Cow,Sheep,Guinea Pig, 产品应用 WB=1:500-2000ELISA=1:500-1000Flow-Cyt=0.2μg /Test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量 51kDa 细胞定位 细胞浆 性状 Liquid 浓度 1mg/ml 免疫原 KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56:S(p-S)PG 亚型 IgG 纯化方法 affinity purified by Protein A 储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed

产品介绍

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular

transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis

via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the

breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal

changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading

to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast

origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin

antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel

of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.