货品编号： Bioss.bs-3770R 品 牌：Bioss 品 名：Anti-Histone H3 (Di Methyl K4)二甲基化组蛋白H3K4抗体 规 格：100ul 产品类型 甲基化抗体 研究领域 肿瘤细胞生物免疫学染色质和核信号转录调节因子表观遗传学 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human,Mouse,Rat,Pig,Cow,Rabbit,Fruit Fly, 产品应用 WB=1:500-2000ELISA=1:500-1000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=0.2μg /TestICC=1:100-500IF=1:100-500（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量 15kDa 细胞定位 细胞核 性状 Liquid 浓度 1mg/ml 免疫原 KLH conjugated synthesised metylpeptide derived from human Histone H3 around the metylation site of Lys4:RT(Di Methyl-K)QT 亚型 IgG 纯化方法 affinity purified by Protein A 储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed

产品介绍 Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of

four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes

different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell

signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20.

Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and

chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both

mitosis and meiosis.