货品编号Bioss.bs-0754R

品牌Bioss

品名Anti-PCNA增殖细胞核抗原抗体

规格100ul

研究领域肿瘤 细胞生物 染色质和核信号 细胞周期蛋白 细胞类型标志物

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量29kDa

细胞定位细胞核

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from mouse PCNA:185-261/261

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a

nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified

by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of

the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein

(cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells.

PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus

40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The

PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic

structure.