货品编号： Sigma.P1585-1MG

品牌：Sigma

品名：PMA

规格：1mg

产品描述：

General description PMA（佛波醇12-十四酸酯13-乙酸酯）是一种蛋白激酶C（PKC）的激活剂和已知的肿瘤促进剂。

PMA还表现出致裂（染色体断裂）活性。[1] Phorbol 12-myristate 13-acetate (PMA) can induce the activity of PLD1 (phospholipase D1) but

not PLD2 (phospholipase D2).[2]

Through the protein kinase C (PKC) signaling pathway, PMA is a tumor promoter associated with:

•gene transcription •cell growth •cell differentiation •programmed cell death •immune pathway •receptor desensitization

Phorbol 12-myristate 13-acetate is used to study:

•growth factors •hormones •cytokines-mediated regulation of cellular growth and differentiation

Application PMA has been used: •for the detection of intracellular cytokines[3] •in CellROX oxidative stress assay[4] •for Huh7 cell stimulation to investigate dipeptidyl peptidase 9 (DPP9) re-distribution[5] •as a positive control in B-cell receptor (BCR) stimulation of chronic lymphocytic leukaemia (CLL) cells[6] •in the differentiation of monocytes (THP -1) into macrophages[7]

适用于 •作为标准品用于分子生物学研究 •肿瘤启动子分析研究 •促进细胞生长

Biochem/physiol Actions Potent tumor promoter; activates protein kinase C (PKC) in vivo and in vitro. 有效的肿瘤促进剂；在体内和体外激活蛋白激酶C。

Features and Benefits •Stimulation of interleukin 2 production of Jurkat cells •T-cell stimulation • Protein kinase C (PKC) activator • NF-κB Activator • Promotion of cell growth • Used in analytical studies of tumor promoters • Molecular Biology grade

Caution 光敏感的，避光保存。

Analysis Note •Stimulation of interleukin 2 production of Jurkat cells •T-cell stimulation • Protein kinase C (PKC) activator •NF-κB Activator •Promotion of cell growth •Used in analytical studies of tumor promoters •Molecular Biology grade