货品编号Bioss.bs-10802R

品牌Bioss

品名Anti-TNF alpha肿瘤坏死因子-α/TNFα/TNF-α抗体

规格100ul

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat,

产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量17/26kDa

细胞定位细胞膜 分泌型蛋白

性 状Liquid

浓 度1mg/ml

免 疫 原Recombinant human TNF alpha protein:77-233/233

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages,

and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic

shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently

linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is

closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and

cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with

interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha

appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of

human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction

of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment

of certain cancers and AIDS Related Complex.