货品编号Bioss.bs-20363R

品牌Bioss

品名Anti-caspase-3 p17 subunit活化半胱胺酸蛋白酶蛋白-3抗体

规格100ul

研究领域细胞生物 免疫学 信号转导 细胞凋亡

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat,

产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量17/32kDa

细胞定位细胞浆

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit :21-120/277

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied

apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed

in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10).

The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active

enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and

activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF).

Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical

studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported

in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been

reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells).

Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated

with neuronal death in Alzheimer's disease.

Reacts with Caspase-3 subunit p17 and precursor.