货品编号: sino biological.16013-HNCH 品 牌:sino biological 品 名:Cardiotrophin 1 Protein, Human, Recombinant 规 格: 5ug 产品描述: Purity
> 85 % as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/ml.
Protein Construction
A DNA sequence encoding the human CTF1 (NP_001321.1) (Ser2-Ala201) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
Accession#
NP_001321.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gly
Molecule Mass
The recombinant human CTF1 comprises 202 amino acids and has a predicted molecular mass of 21.2 kDa. The apparent molecular mass of the protein is
approximately 26.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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