货品编号： Thermo.11960044 品 牌：Thermo 品 名：gibco DMEM, high glucose, no glutamine 规 格： 500 mL /瓶

Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture 产品描述： DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell

lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the

right formulation using the media selector tool.

This DMEM is modified as follows:With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES

The complete formulation is available.

Using DMEM DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal

Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose

levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation,

commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10%

CO2 environment to maintain physiological pH.