货品编号： Thermo.21217 品 牌：Thermo 品 名：Thermo Scientific EZ-Link Sulfo-NHS-Biotin 规 格： 50mg 产品描述： Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other

molecules that have primary amines.

Features of EZ-Link Sulfo-NHS-Biotin:

•Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes •Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes •Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides •Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds •Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved •Very short—spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.

Sulfo-NHS-Biotin is the shortest of three very similar EZ-Link Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation

of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common

application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three Sulfo-NHS-ester

reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-

Biotin is offered in several package sizes and as complete protein labeling kits.