货品编号： thermo.11456D 品 牌：thermo 品 名：Gibco Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation 规 格： 0.4 mL 产品描述： Dynabeads® Mouse T-Activator CD3/CD28 is for the activation and expansion of mouse T-cells for research purposes.

With Dynabeads® Mouse T-Activator CD3/CD28 you get:

• Activation of T-cells without the need of feeder cells • Activated cells that retain in vivo-like function • Maximal reproducibility without contamination by soluble antibodies or mitogens • From 100- to 1000-fold T-cell expansion

How Dynabeads® Mouse T-Activator CD3/CD28 works:

Dynabeads® Mouse T-Activator CD3/CD28 offers a simple method for activation and expansion of T-cells that does not require

feeder cells (antigen-presenting cells) or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to

antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary

and co-stimulatory signals, optimized for efficient T-cell activation and expansion. Expansion of the T-cell population can be stimulated

using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.

For expansion of antigen specific T-cells from T-cell clones or T-cell lines, we recommend using Dynabeads® Mouse T-Activator

CD3/CD28/CD137.

Starting samples Start with single cell suspensions from spleen or lymph nodes, mononuclear cells (MNCs), whole blood, or T-cell subsets including CD3+

T cells, CD4+ or CD8+ T cells, or regulatory T cells. Get an overview of the different Dynabeads® available for cell isolation.

Downstream applications The activated T-cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene

expression, etc.). T-cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.