货品编号：bioss.bs-0892R

品牌：

品名：Rabbit Anti-Bad antibody

规格：50ul

研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 新陈代谢

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat,

产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量18kDa

细胞定位细胞浆 细胞膜

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human Bad:120-204/204

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins

Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and,

once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad

represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the

apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced

phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction

is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the

promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.